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HEPATITIS B VIRUS CORE PROMOTER MUTATIONS AND THEIR RELATEDNESS TO GENOTYPES AND LIVER DISEASE SEVERITY IN HBV-INFECTED CHRONIC ISOLATES FROM THE STATE OF HARYANA IN INDIA. Vikas Pahal, Jasbir Singh

HEPATITIS B VIRUS CORE PROMOTER MUTATIONS AND THEIR RELATEDNESS TO GENOTYPES AND LIVER DISEASE SEVERITY IN HBV-INFECTED CHRONIC ISOLATES FROM THE STATE OF HARYANA IN INDIA.

Vikas Pahal, Jasbir Singh

International Journal of Applied Microbiology Science 2014; 3(3):1-11

Abstract
There are several reports throughout the world which demonstrated that Hepatitis B virus (HBV) core promoter (CP) mutations, known to be linked to either the disease status or to the genotypes, could have an impact on the progression and severity of liver disease in HBV-infected patients. So, we decided to find out the association, if any, of various indigenous HBV core promoter mutations with HBV genotypes and severity of liver disease from this region of the world. Present study was conducted on three main categories of patients: chronic asymptomatic or inactive carriers, chronic symptomatic carriers with HBV ‘e’ antigen (HBeAg) marker, and chronic symptomatic carriers without HBeAg marker from the state of Haryana in north India. Some acute cases and hepatocellular carcinoma cases (HCC) were also studied. Core promoter region (1661-1921) specific primers were used to amplify the CP region and directly sequenced to analyze the presence of various mutations. The results revealed the significant association of CP mutations like 1762/1764(T/A), 1809(T) and 1812(T) with genotype A, and 1809(G) and 1812(C) with genotype D. Some mutations like 1695(K), 1753(C), 1762/1764 (A/G) and 1809(T) were found significantly with HBeAg positive marker HBV strains. Similarly, it was also observed that some mutations like 1695(K), 1698(W) and 1699(K) were found frequently in chronic symptomatic group, whereas mutations like 1809(G) and 1812(C) observed in chronic asymptomatic group. In conclusion, various CP mutations were found to have statistically significant association with different clinical categories and to HBV-genotypes.

Key words: Core promoter mutations, genotype, HBV
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