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IN VITRO COMPARATIVE ANTIMICROBIAL EFFICACY OF EMBLICA OFFICINALIS FRUIT EXTRACTS AGAINST SOME GRAM POSITIVE AND GRAM NEGATIVE BACTERIA. Satyajit G Patil, Deshmukh AA, Amol R Padol, Jagadale AA

IN VITRO COMPARATIVE ANTIMICROBIAL EFFICACY OF EMBLICA OFFICINALIS FRUIT EXTRACTS AGAINST SOME GRAM POSITIVE AND GRAM NEGATIVE BACTERIA.


Satyajit G Patil, Deshmukh AA, Amol R Padol, Jagadale AA

International Journal of Applied Microbiology Science 2013; 2(2):1-6

Abstract

A study was carried out to assess and compare the antibacterial activity of aqueous and organic viz. acetone, chloroform, ethyl acetate and methanol extracts of Emblica officinalis (EO) fruit against pathogenic strains of Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pasteurella multocida by disc diffusion method. The aqueous, acetone, ethyl acetate and methanol extract were found to be effective against all the microorganisms under test where as chloroform extract was effective against E. coli and P. multocida but S. aureus and K. pneumoniae were found to be resistant. The acetone fruit extract had shown maximum activity against E. coli and P. multocida, whereas the aqueous fruit extract was more effective against K. pneumoniae and the methanol fruit extract against S. aureus. The positive control ciprofloxacin used in the study was more effective in inhibiting the growth of all tested organism compared to all the extracts of EO fruit. The study concluded that the E. officinalis fruit have potent antibacterial activity against both Gram positive and Gram negative bacteria and the aqueous, acetone, ethyl acetate and methanol extracts have more potent activity than that of chloroform extract.

Keywords: Antibacterial activity, disc diffusion, Emblica officinalis fruit, zone of inhibition
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