HIGH QUALITY SCIENTIFIC CONTENT,  ONLINE OPEN ACCESS,  GLOBAL SCIENTIFIC AUDIENCE,  COST EFFECTIVE, ANY ONE CAN DOWNLOAD REPRINTS 
Spreading knowledge

Google Scholar

Google Scholar

Archives

Back 

MEDIA OPTIMIZATION USING ORTHOGONAL ARRAY TECHNIQUE FOR CHOLESTEROL OXIDASE PRODUCTION BY STREPTOMYCES SP. Parekh SN, Desai PB

MEDIA OPTIMIZATION USING ORTHOGONAL ARRAY TECHNIQUE FOR CHOLESTEROL OXIDASE PRODUCTION BY STREPTOMYCES SP.

Parekh SN , Desai PB

International Journal of Applied Microbiology Science 2012;1(2);32-43.


Abstract
The present study focuses on optimization of media components to enhance cholesterol oxidase (CHO) production by Streptomyces sp. isolated from waste of regional oil mill using Taguchi orthogonal array design. One factor-at-a-time method was used to investigate the effect of carbon sources, nitrogen sources and initial pH on biomass growth and enzyme production. A three-level Taguchi orthogonal array design of L9 (34) was employed to select the effect of medium components on cholesterol oxidase production. The optimum composition of fermentation medium as obtained by statistical analysis was starch 2 % w/v, peptone 0.9 % w/v, yeast extract 0.3 % w/v, Tween 80 0.1 % v/v and employed for cholesterol oxidase production. The optimized medium exhibited 1.76-fold increase in enzyme activity as compared to the non-optimized medium (2.82 U/ml and1.6 U/ml, respectively) at shake flask level.

Keywords: Cholesterol oxidase optimization, Streptomyces sp., Taguchi orthogonal array design.
Attachments:
Time to create page: 0.13 seconds


Copyright © All rights reserved Science Instinct Publications sciipub.com