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PHYLOGENETIC ANALYSIS OF LIPASE PRODUCING BACTERIA USING PHYSIOLOGICAL AND MOLECULAR TECHNIQUES FROM THE LONAR CRATER. Tambekar DH, Dhundale VR

PHYLOGENETIC ANALYSIS OF LIPASE PRODUCING BACTERIA USING PHYSIOLOGICAL AND MOLECULAR TECHNIQUES FROM THE LONAR CRATER.

Tambekar DH, Dhundale VR

International Journal of Applied Microbiology Science 2012;1(2); 24-31.

Abstract
Microbial lipases are one of the most important group of enzymes with applications in detergents, manufacture of food ingredients and pitch control in pulp and paper industry. Lipases have interesting role as biocatalysts in organic media. Culture dependent phenotypic characterization analysis was done to study the lipase producing bacteria from the Lonar crater. The uniqueness of the Lonar Lake water is its salinity and alkalinity. Aerobic, haloalkalophilic bacteria were isolated and characterized from sediment and water samples of Lonar Lake. Fifty five bacterial strains were isolated using different enrichment media. Lipolytic enzyme producing thirteen bacterial strains were selected on the basis of their lipolytic activities and subjected to physiological and 16S rRNA gene sequencing analyses for their identification. Phylogenetic position revealed that bacterial strains were related to phylum Firmicutes and belongs to the genera Bacillus; the species were Bacillus flexus, Bacillus pseudofirmus, Bacillus pumilus, Bacillus halodurans, Bacillus cereus and Bacillus sp. All these bacterial strains were obtained after culturing them within a pH range of 7–12 and a temperature range of 37–50°C, with an optimum growth at pH 10 and 45°C for most of the strains. These bacterial strains may have biotechnological potential by being useful for the production of lipase .

Keywords: Lonar crater, Soda lake, Molecular phylogeny, Bacillus, Lipase
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