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EVALUATION OF AMYLASE ACTIVITY OF THE AMYLOLYTIC FUNGI ASPERGILLUS NIGER USING CASSAVA AS SUBSTRATE. S Siva Sakthi, D Kanchana, P Saranraj, G Usharani

EVALUATION OF AMYLASE ACTIVITY OF THE AMYLOLYTIC FUNGI ASPERGILLUS NIGER USING CASSAVA AS SUBSTRATE.

S Siva Sakthi, D Kanchana, P Saranraj, G Usharani

International Journal of Applied Microbiology Science 2012; 1: 24-34

Abstract

Fungal amylases are used for hydrolyzing carbohydrate, protein and other constitutes of soybeans, wheat into peptides, amino acid, sugars and other low molecular weight compounds. The present study was carried out to evaluate the amylase activity of the amylolytic fungi Aspergillus niger using cassava waste as a feed substrate. The fungi were isolated from the soil samples and the fungi isolated were identified as Aspergillus niger based on Lactophenol cotton blue staining and plating on appropriate fungal growth medium. Amylase production by Aspergillus niger was detected by the disappearance of blue colour in the Starch agar medium around the microbial colonies after incubation. Cassava was used as the substrates for the amylase production. Solid state fermentation was carried out for the production of amylase using Aspergillus niger. The effect of different carbon sources, nitrogen source, Temperature and pH was determined on enzyme production by Aspergillus niger. Amylase activity was determined by four methods such as DNSA method, Dextrinizing activity method, decrease in Starch-iodine color intensity and Plate assay.

Key words: Amylase, Aspergillus niger, Cassava and Solid state fermentation.
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