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ISOLATION AND CHARACTERIZATION OF β-SITOSTEROL FROM LEAF METHANOL EXTRACT OF SVENSONIA HYDEROBADENSIS – A RARE MEDICINAL PLANT TAXON M Lingarao, N Savithramma

ISOLATION AND CHARACTERIZATION OF β-SITOSTEROL FROM LEAF METHANOL EXTRACT OF SVENSONIA HYDEROBADENSIS – A RARE MEDICINAL PLANT TAXON

M Lingarao, N Savithramma

International Journal of Natural Product Science 2013; 3(1): 1-7

Abstract

Phytochemical screening of the methanolic leaf extract of Svensonia hyderobadensis revealed that the presence of steroids, terpenoids, phenolic compounds, saponins, tannins and flavonoids. The aim of the present study was to isolate and characterize the bioactive compound from S. hyderobadensis a rare medicinal plant. Dried leaf powder of S. hyderobadensis was extracted with various solvents and subjected to column chromatography and characterized by IR, LC-MS, 1H NMR and 13C NMR data analysis. The results were obtained by methanolic and chloroform solvent system (9:1), based on the spectral data analysis and chemical reactions, the compound have been established as β-sitosterol.

Keywords: Svensonia hyderobadensis, β-sitosterol, leaf, chromatography
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