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PHYTOCHEMICAL SCREENING, CHROMATOGRAPHIC EVALUATION AND ANTIBACTERIAL ACTIVITY ON STEM BARK EXTRACTS OF MITRAGYNA INERMIS. Tor-Anyiin TA , AM Orokpo

PHYTOCHEMICAL SCREENING, CHROMATOGRAPHIC EVALUATION AND ANTIBACTERIAL ACTIVITY ON STEM BARK EXTRACTS OF MITRAGYNA INERMIS.

Tor-Anyiin TA , AM Orokpo

International Journal of Natural Product Science 2012; 2(3): 45-52


Abstract

Mitragyna inermis is a plant used ethnomedically, against bacterial infections and for pain relief. Its stem bark was successively extracted with peterolum ether and ethanol. Dried crude ethanol extract was dissolved in water, filtered and filtrate partitioned successively with chloroform, ethyl acetate and n-butanol to give fractions CMI, EAMI and BMI, for chloroform, ethyl acetate and n-butanol, respectively. Phytochemical screening of stem bark extract/fractions revealed presence of alkaloids, tannins, flavonoids, terpenoids and resins. Presence of these secondary metabolites was further evaluated by thin layer chromatography (TLC) using colours and Rf values. Crude ethanol extract, water insoluble residue and fractions were investigated for their antibacterial activity against Staphylococcus aureus, Escherichia coli, Streptococci pyogenes, Salmonella typhi and Proteus mirabolis. Ethanol crude extract, water insoluble residue and ethyl acetate fraction did not inhibit growth of E coli, but inhibited growth of S aureus, P mirabolis, S pyogenes and S typhi. n-Butanol fraction failed to inhibit growth of S. aureus but inhibited growth of other bacteria tested. Except S. pyogenese, all tested bacteria were inhibited by residue of water soluble crude ethanol extract.

Key words: Antibacteria activity, Mitragyna inermis, TLC, phytochemical screening, sterm bark extracts.
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