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KAEMPFEROL: THE ANXIOLYTIC CONSTITUENT OF ACTAEA SPICATA L. Sonali Batra, Reecha Madaan, Suresh Kumar and Anupam Sharma

KAEMPFEROL: THE ANXIOLYTIC CONSTITUENT OF ACTAEA SPICATA L.

Sonali Batra, Reecha Madaan, Suresh Kumar and Anupam Sharma

International Journal of Natural Product Science 2012: Spl Issue 1:228.

Abstract(RBIP-228)

Our previous investigations have shown that methanol extract of Actaea spicata L. (Ranunculaceae) roots and its polyphenol fraction exhibited significant antianxiety activity. The present investigations were undertaken with an objective to isolate anxiolytic constituent(s) from the plant following antianxiety activity-directed-fractionation using standard chromatographic techniques. The column chromatography of polyphenol fraction, obtained from methanol extract of A. spicata roots, yielded six sub-fractions (F1-F6). These sub-fractions, separately suspended in a suitable vehicle, were administered orally to mice, and the activity was compared with that observed in the control group as well as with the group treated with the standard anxiolytic drug diazepam. Among the sub-fractions tested, maximum anxiolytic activity was observed in the F5 at the dose of 10 mg/kg, p.o. which was at par with that of diazepam as is evident from statistical equivalence between the results of this dose and that manifested by diazepam. The column chromatography of bioactive F5 yielded three subfractions F5.1, F5.2 and F5.3. A compound (AR1; 1.426 g) separated as white amorphous powder during the process of preparing polyphenol fraction from methanol extract of A. spicata roots. The yellow star like crystals of AR2 (102 mg) were obtained from sub-fraction F4. The column chromatography of bioactive sub-fraction of F5 yielded AR3 (126 mg) as needle shaped crystals from F5.2. These constituents, separately suspended in a suitable vehicle, were administered orally to mice, and the antianxiety activity was compared with that observed in the control group as well as with the group treated with the standard anxiolytic drug diazepam. Among the isolates tested, maximum anxiolytic activity was observed in the AR3 at the dose of 2 mg/kg, p.o. which was statistically equivalent to the one exhibited by diazepam. The structure of AR3 was elucidated by UV, IR and NMR data and characterized as kaempferol. It is concluded that kaempferol is responsible for anxiolytic effects of A. spicata.

Keywords: Actaea spicata, Anxiolytic, Kaempferol, Ranunculaceae
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