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ANALYTICAL METHOD DEVELOPMENT FOR SIMULTANEOUS ESTIMATION OF METFORMIN AND GLIPIZIDE IN COMBINED DOSAGE FORM. Jasmine Chaudhary, Akash Jain and Vipin Saini

ANALYTICAL METHOD DEVELOPMENT FOR SIMULTANEOUS ESTIMATION OF METFORMIN AND GLIPIZIDE IN COMBINED DOSAGE FORM.

Jasmine Chaudhary, Akash Jain and Vipin Saini

International Journal of Natural Product Science 2012: Spl Issue 1:102.

Abstract(RBIP-102)

Objective: To develop simple, rapid and accurate spectrophotometric method for the estimation of metformin and glipizide which can be applied successfully for the analysis of both the drugs in pure as well as in their combined dosage form.

Material and Method: A double-beam Shimadzu UV-Visible spectrophotometer, 1800, with a pair of 1cm matched quartz cells was used to measure the absorbance of the solutions in developed simultaneous equation method. Methanol was selected as solvent for the preparation of solutions. After scanning 10μg/ml solution of each drug separately in the range of 200-400 nm wavelength of maximum absorption of metformin (237 nm) and glipizide (229 nm) were selected for simultaneous equation method. The method was validated statistically as per ICH guidelines.

Results: Linearity ranges from 5-30 μg/ml for both drugs. %RSD calculated was less than equal to 2 which indicates good accuracy and reproducibility of the method. The recovery studies indicate that these drugs could be quantified simultaneously and that there is no interference of the excipients present in the formulation.

Conclusion: The developed UV spectroscopic method is simple, precise, less time consuming, economical and accurate and thus suitable for the analysis of metformin and glipizide in the bulk and tablet dosage form without the interference of excipients.
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