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ANTI-FUNGAL ACTIVITY OF NANOEMULSION BASED AMPHOTERICIN-B AGAINST ASPERGILLUS NIGER AND CANDIDA ALBICANS. Aninder Singh, Abdul Faruk, Afzal Hussain, Gurpreet singh, Davinder Singh

ANTI-FUNGAL ACTIVITY OF NANOEMULSION BASED AMPHOTERICIN-B AGAINST ASPERGILLUS NIGER AND CANDIDA ALBICANS.

Aninder Singh, Abdul Faruk, Afzal Hussain, Gurpreet singh, Davinder Singh

International Journal of Natural Product Science 2012: Spl Issue 1:78.

Abstract (RBIP-78)

The main objective of the study was to evaluate antifungal activity of nanoemulsion and its comparison with marketed Fungisome cream®. In present study an attempt has been made to develop cost effective, non-nephrotoxic and thermodynamic stable formulation using spontaneous titration method followed with sonication method, evaluated antifungal activity using Agar well diffusion method. Two strains of fungi Aspergillus niger 989×105 (CFU/ml) and Candida albicans 473×105 (CFU/ml) were subcultured. The stock culture was maintained at 40C on slope of 3.5% Sabouraud dextrose agar media. The antifungal activities of different concentration of the nanoemulsion and elastic liposome prepared shows that the nanoemulsion has strong antifungal activity against Candida albicans with MIC values range of 05 µg/ml-500 µg/ml and inhibition zone of 2.6-15.9 mm after 24 hrs whereas others have MIC values 50.5µg/ml. The result shows the inhibitory activity of drug solution (DMSO) within concentration range of 50µg ml-1-500µg ml-1 against both strains whereas nanoemulsion shows potential inhibitory action in the dilution range of 5µg ml-1-500µg ml-1 against the same both strain Candida albicans and Aspergillus niger at 48 hrs.
The optimized nanoemulsion was found to have minimum MIC values 50.5µg/ml at 48 hrs as compared to the commercial formulation. The MIC of the nanoemulsion against Candida albicans and Aspergillus niger was more potent than marketed reference and positive control, while liposome was less than the same. The result demonstrates that nanoemulsion formulation has potential antifungal activity than commercial and liposomal formulation against Candida albicans and Aspergillus niger.

Key words: MIC, Amphotericin-B, Nanoemulsion, Zone of inhibition
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